Cell & Tissue Analysis Core
The Cell and Tissue Analysis Core will accomplish three aims:
- To provide service, assistance, and consultation in cell and tissue analysis.
- To provide comprehensive cell and tissue analysis.
- To provide Cell line authentication and mycoplasma testing service.
Cell and Tissue Analysis (CTA) core acknowledges that unraveling the mechanisms governing tumor growth, progression, and responses to therapy requires a multidimensional approach encompassing comprehensive cell and tissue analysis. By offering cutting-edge technologies and expert guidance, Core empowers cancer researchers to embark on a rigorous scientific journey. The premise is straightforward: a deeper understanding of cancer biology can be achieved when we possess the tools to analyze, quantify, and comprehend cellular and tissue level complexities.
Equipment
- Veridiam TMA construction system
- Leica Bond RX automated Immunohistochemistry system
- Leica Bond III automated Immunohistochemistry system
- Leica ST5020 Multistainer instrument
- Leica CV5030 Cover Slipper
- Nikon Ni Microscope System
- Olympus CK56 inverted microscope system
- Biorad T100 PCR machine
- Bio-Rad CFX 96 Realtime PCR machine
- BioRad QX100 Droplet Digital PCR System
- Seahorse XFe96 Metabolic analyzer
- Baker Ruskinn InvivO2 400 Hypoxia Workstation x 2Rare cell ISET CTC isolation system
- Applied Biosystems 3500 XL Genetic analyzer
Provided Services
- Tissue Microarray (TMA) Construction: Enables high-throughput evaluations of cancer biomarkers by concurrently analyzing various tissue samples. Our core can construct tissue microarrays with up to 72 cores per block.
- Mammalian Cell Lines Array Construction Service: This service accelerates the concurrent screening of multiple cell lines, thus expediting research into cellular behavior and responses.
- Rare Cell ISET CTC Isolation System: This cutting-edge technology facilitates the isolation and characterization of circulating tumor cells (CTCs), allowing novel research directions in cancer dynamics and therapeutic response.
- Immunofluorescence Staining: Provides single to quad immunofluorescence staining, enabling researchers to simultaneously visualize multiple markers, decoding intricate cellular interactions and signaling.
- Specialized Staining: Includes staining methods such as Periodic acid-Schiff, Trichrome, Safranin O, and VVG Elastic staining to deliver in-depth tissue insights.
- TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling): Used for detecting DNA fragmentation, enabling apoptosis evaluation and understanding cell death mechanisms.
- RNAscope (Chromogen/Fluorescence), miRNAscope, and BaseScope Analyses: Advanced techniques for detecting specific RNA molecules, revealing key gene expression trends and regulatory pathways.
- Seahorse Flex Analyzer: Enables metabolic flux assessments to understand cellular metabolism's role in cancer evolution. Please refer to Figure 2 for an illustrative study conducted using this system.
- Hypoxia Workstation. The Core provides two Baker Ruskinn InvivO2 400 Hypoxia Workstations for rigorous in vitro as well as in vivo studies on tumor hypoxia.
- Bio-Rad QX100 Droplet Digital PCR System: Provides precise and sensitive digital PCR analysis. Refer to Figure 3 for a demonstration of this system in studying copy number variations of a specific gene.
- Human Cell Line Authentication and Mycoplasma Testing: Ensuring the utmost reliability of cell-based experiments, Core conducts STR-based cell line authentication and PCR-based mycoplasma testing, underpinning the integrity of research outcomes.
Core Directors
Core Director
Muralidharan Jayaraman, PhD
Muralidharan-Jayaraman@ouhsc.edu
Core Co-Director
Kar-Ming Fung MD PhD
KarMing-Fung@ouhsc.edu
Oversight Advisory committee
Priyabrata Mukherjee, PhD
Priyabrata-Mukherjee@ouhsc.edu
Resham Bhattacharya, PhD
Resham-Bhattacharya@ouhsc.edu
Min Li, PhD
Min-Li@ouhsc.edu
Usage Acknowledgement
If research supported by Stephenson Cancer Center core facilities results in a publication or news release, please acknowledge this support in your manuscript. The acknowledgement can be, “The --- service provided by the MTCRO Cell and Tissue Analysis Core was supported partly by the National Institute of General Medical Sciences Grant P30GM154635 and National Cancer Institute Grant P30CA225520 of the National Institutes of Health.” Following publication, please send us one of your reprints for our records.
Pricing
Please contact Core for pricing structure. We will provide the detailed breakdown of charges for each service.
